I-2- [i-tris (i-hydroxymethy) i-methylaminio] -1-ethanesfonic acid; I-COS.: 7365444414-8 umfanekiso

I-2- [i-tris (i-hydroxymethy) i-methylaminio] -1-ethanesfonic acid; I-COS.: 7365-8-8

Inkcazo emfutshane:

Igama leekhemikhali:I-2- [i-tris (hydroxymethy) i-methylaminio] -1-ethanesfonic acid
I-CAY NO .:7365-44-8-8-8-8-8
Ifomula yemolekyuli:C₆H₁₅NO₆S
Ubunzima bemolekyuli:229.254
Ikhowudi ye-HS;29221980
Ifayile ye-MOL:7365-44-8.mol

Thumela i-imeyile kuthi

Iinkcukacha zemveliso

Iimpawu zeMveliso

I-2- [i-tris (Hydroxymethy) I-methylaminio] -1-ethanesfonic acid 73654444

Izithethantonye: I-Chebi: 39036; 2 - {[2-Hydroxy-1,1-Bis (Hydroxymethy; N-Tris (Hydro I-Xymethyl

Ipropathi yekhemikhali ye-2- [i-tris (i-hydroxymethy) i-methylaminio] -1-ethanesfonic acid

● Inkangeleko / umbala: Umgubo we-crystalline emhlophe
● Inqaku lokunyibilika: ~ 223-225 ° C
● Isalathiso seNgxelo yeNgxelo: 1.57
● I-PKA: 7.5 (nge-25 ℃)
● I-PSA: 135.47000
● Ukuxinana: 1.554 g / cm3
● I-LGPP: -1.34880

● Indawo yokugcina: Ivenkile yeVenkile ye-RT.
● Ukuzalelwa. :H2O: 1 m nge-20 ° C, icacile, ayinabala
● Ukunyibilikisa amanzi. Inyibiliki
● I-XlogP3: -5.8
● I-Hydrogen Bop Count Count: 4
● Ubalo lwe-Hydrogen Bopptor: 6
● I-Count Chot Rot: 6
● Ubunzima obuthe ngqo: 229.06200837
● I-Atom I-Atom Com: 14
● Ukuntsonkotha: 220

Ulwazi olukhuselekileyo

● I-pictogram (s): Imveliso (2) Xi
● Iikhowudi zengozi: xi
● Iingxelo: 36/37/38
● Iingxelo zoKhuseleko: 22-24 / 25-36-26

Iluncedo

● I-canonical iyancuma:C (CS (= o) (= o) [o) [o -]) [NH2 +] C (CO) (CO) CO
● Ukusetyenziswa:I-analog yesakhiwo kwi-tris buffer. Buffer buffer. I-Tes yiarhente esetyenziswayo esetyenziswayo.

Intshayelelo Eneenkcukacha

I-2- [i-tris (hydroxymethy) i-methylaminio] -1-ethanesfonic acid, ekwabizwa ngokuba yi-Tes okanye i-N-2-HydroxyylPiperazine-I-Ethanesfonic Acid, yi-Zwiterinic Acid isetyenziswa rhoqo kwi-biology yebhayoloji ye-biochemical kunye nemolekyuli.
Tesihlala isetyenziswa njenge-buffer evavanyiweyo yelebhu ngenxa yokukwazi kwayo ukugcina imeko ye-PH ye-PH, ngakumbi kuluhlu lwe-6.5 ukuya kwi-8.5. Ikwanayo i-Inraviot ephantsi ye-Ultraviot yokukhanya, eyenza ilungele ukusetyenziswa kwizicelo ze-spectraphotometric. I-InEt i-Inert kwaye ayiphazamisi iimvavanyo ezininzi zokufunda, zivumela iimvavanyo ezinokuthenjwa, zivumela iimvavanyo ezinokuthenjwa.
Tesisetyenziswa rhoqo kwii-oscays zebhayoloji ne-biochemical, ukucocwa kweprotein, i-electrophoresis, kunye nee-Media zeNkcubeko.
Kufanelekile ukuba uqaphele ukuba i-tis i-hygroscopic, okuthetha ukuba ifumana ngokulula umswakama kwindalo esingqongileyo, ngoko kubalulekile ukugcina nokuyisebenzisa ngokufanelekileyo ukugcina ukusebenza kwayo.

Isicelo

I-2- [i-tris (i-hydroxymethy) i-methylaminio] -1-ethanesfonic acid yi-acid efanelekileyo (i-TES) yi-commaund efanelekileyo. Ezinye zosetyenziso lwayo eziphambili zezi:
Iarhente ye-Bufferty:I-Tes ihlala isetyenziswa njengecandelo le-buffting kwi-biochemical kunye neemolekyuli zebhayoloji. Inceda ukugcina imeko ye-p ye-p ye-p ye-ph eqinileyo, ngakumbi kuluhlu lwe-6.5 ukuya kwi-8.5
I-Biological kunye ne-biochemical Asseys:I-Tes isetyenziswa kwi-biological eyahlukeneyo yebhayoloji ne-biochemical apho kuhlala i-H ye-PH yephumo elipheleleyo kubalulekile. Kuyanceda ukugcina i-PH yesisombululo isisiseko kwaye inika uzinzo kwiimeko zokuphendula.
Ukucoca iiprotein:Ii-Tes zihlala zisetyenziswa kwiinkqubo zokucoca iiprotein ngenxa yobume bayo bokukrazula kunye nokuhambelana nemisebenzi ye-enzyme. Kuyanceda ukugcina uzinzo kunye nomsebenzi weeproteni ngamanyathelo okucoca.
I-electrophoreris:I-Tes iqeshwe njengecandelo le-buffting kwi-gel ye-gel electrophoressis, ngakumbi kwi-Polyacybusside gel Electrophoreris (iphepha). Inika indawo ezinzileyo ye-hle yokwahlulahlula kunye nokufuduka.
I-Ints yeCal yeSelfowuni:I-Tes ibandakanyiwe kwimithombo yeeMgadi zeSoMeli ukugcina i-PH ye-PH yokukhula kweseli ngokugqibeleleyo kunye nokwanda. Kuyanceda ukuzinzisa i-PH yenkcubeko kwaye kubonelela ngendawo efanelekileyo yokwezazi.
Kubalulekile ukuba uqaphele ukuba isicelo esithile kunye nokugxininiswa kwee-Tes kungahluka ngokuxhomekeka kwiimfuno zovavanyo. Ukuphatha ngokufanelekileyo, ukugcinwa, kunye nokuqwalaselwa kweenkcukacha ezichazwe kumaxwebhu obuchwephesha bemveliso kubalulekile ukuqinisekisa ukusebenza nokhuseleko lwayo.