Iimveliso
I-3-morpholinoufropasulpanininic acid; I-Cas No.: 1132-61-2
Izithethantonye: I-3-morpholin-4-ium-i-4-ylpropane-1-i-Chebi: i-3- (i-3-
Ipropathi yamachiza e-3-morpholinphopherropanesifoanic acid
● Inkangeleko / umbala: ipowder emhlophe
● Uxinzelelo lwe-vapor: 0pa ngo-25 ℃
● Inqaku lokunyibilika: 277-282 ºc
● Isalathiso seNgxelo yeNgxelo: 1.512
● I-PKA: 7.2 (nge-25 ℃)
● Inqaku leFlash: 116 ºc
● psa:75.22000
● Ukuxinana: 1.298 g / cm3
● I-LOGP: 0.61520
● Indawo yokugcina indawo.
● Ukuzalelwa. :H2O: 1 m nge-20 ° C, Cacisa
● Ukuzola amanzi
● I-xlogP3: -3.2
● I-Hydrogen Bop Count Count: 1
● Ubalo lwe-Hydrogen Bopptor: 4
● I-Count Count Count Count: 3
● Ubunzima obuthe ngqo: 209.07217913
● I-Atom I-Atom Com: 13
● Ukuntsonkotha: 214
Ulwazi olukhuselekileyo
● I-pictogram (s):
Xi
● Iikhowudi zengozi: xi
● Iingxelo: 36/37/38
● Iingxelo zoKhuseleko: 26-36
Iluncedo
I-Canonical iyancuma:I-C1COCC [NH +] I-1CCCS (= o) (= o) [o)
Inkcazelo:I-MOPS (i-3-morpholinphopherropanenic acid) yi-buffer engeniswe yi-et elungileyo et al. Ngo-1960s. Yinto yokufakelwa kwesakhiwo kwi-mes. Isakhiwo sayo sekhemikhali siqulathe iringi ye-morpholine. I-Hepes yindawo efana ne-probff emnandi equlathe i-piperazine ring. Nge-PKA ye-7.20, ii-mops zezinto ezibalaseleyo zeenkqubo ezininzi zebhayiloji kwi-Ph.it isetyenziswa njengearhente ye-bufferity ye-bhuti ngaphantsi kwe-PH 7.5.
IISEBE:I-3- (n-morpholino) i-propaseulfonic acid okanye i-mops ngenxa ye-proper ye-biechel ye-biechemical kwi-roochel ye-roochel ye-roswell ye-roswell ye-roswell ye-roswell ye-roswell ye-roswell (i-RPM) ye I-Linkeng Fungal Inoculum.as i-buffer kwi-Copillary-Electrophoresis ukuya kwi-Advation yokuSebenza.Cols ye-proterins evela kwiisampuli ze-ALGAL. I-MOPS isebenza njenge-arhente yeenjongo ezininzi ezisetyenziselwa uphando olwahlukeneyo lwebhayoloji. I-MOPS isetyenzisiwe njenge: I-Insopt yeSongezelelo kwiNkcubeko kwi-Lentivicle ivelise iarhente ye-buffer kwindawo yokukhula kwe-nucleial
Intshayelelo Eneenkcukacha
I-3-morpholinOpropanesifoanic acid, ekwabizwa ngokuba yi-MOPS, yindawo yemichiza esetyenziswa rhoqo njengearhente ye-buffer kwizicelo ezahlukeneyo zebhayiloji kunye ne-biochemical. Yinto yomgubo omhlophe we-crystalline enyibilikayo emanzini.
I-MOPS inefomula yekhemikhali ye-C7H15NO4s kunye nobunzima bemolekyuli ye-209.26 g / mol. Inexabiso le-PKA malunga ne-7.2, esenza i-buffer esebenzayo yokugcina i-ph ye-In kwi-7.0-7.5.
Olunye losetyenziso oluphambili lwe-mops kwi-molecular biology kunye ne-biochemistry uphando lokulawula i-ph kwi-assulos ezahlukeneyo zebhayiloji kunye novavanyo. Ihlala isetyenziswa kwizicelo ezinjenge-DNA / RNA yodwa, ukukhutshwa kweprotein, ukuphendula okuqinisekileyo, kunye ne-gel electrophoreris. I-MOPS ibonelela ngolawulo oluzinzileyo nolungabuhlobo, ivumela abaphandi ukuba bagcine iimeko ezifanelekileyo zovavanyo lwabo.
I-MOPS inokusetyenziswa njenge-buffer kwiiseli zenkcubeko kunye neendlela zeChromatography. Yaziwa ngokuba yi-Anraviolet yayo ye-Ultraviolet (UV) kuluhlu lwe-240-300 NM, iyenza ilungele ukusetyenziswa kwezicelo ze-UV.
Xa usebenza ne-MOPS, kubalulekile ukuyisingatha ngononophelo olufanelekileyo. Ifanele igcinwe kwindawo epholileyo, eyomileyo kwaye igcinwe kude kwizixhobo ezingahambelaniyo. Amanyathelo okhuseleko, njengokunxiba izixhobo ezifanelekileyo zokukhusela, kufuneka zilandelwe xa zithathwa le computha.
Njengayo nayiphi na imichiza, kuyacetyiswa ukuba uphonononge iphepha ledatha yoKhuseleko (i-SDS) kwaye inxibelelane nomenzi okanye oqeqeshiweyo ukuqhuba imiyalelo ethile kunye nemiyalelo yokusetyenziswa.
Isicelo
I-3-morpholinphopherropanisenespaninic acid (Mops) ineentlobo ngeentlobo zezicelo ezahlukeneyo. Ezinye iindlela eziqhelekileyo zibandakanya:
Iarhente ye-Bufferty:I-MOPS isetyenziswa rhoqo njengearhente ye-buffer kwi-biologizy kunye neemvavanyo ze-biochemical. Inceda ukugcina i-PH i-PH kwizisombululo, ngakumbi kwi-ph ye-ph ye-7.0-7.5
I-biology biology kunye ne-biochemists: I-MOPS isetyenziswa ngokubanzi njenge-buffer kwi-DNA / I-RNA yokuZahlula, ukukhutshwa kweprotein, i-enccolmatic yokuphendula, kunye ne-gel electrophoreris. Inika iimeko ezifanelekileyo zeZithandelo kwaye ziqinisekisa iziphumo ezinokuthenjwa.
I-Ints yeCal yeSelfowuni: I-MOPS isetyenziswa njengearhente ye-buffer kwimithombo yeendaba ye-Ints. Inceda ukugcina i-PH ngaphakathi kuluhlu olufunayo, ibonelela ngendawo yokuhlala yokukhula kweseli nokwenzakala.
I-chromatography:I-MOPS isetyenziswa njenge-buffer kwiindlela ezahlukeneyo ze-chromatography, kubandakanya i-chromatography kunye nobungakanani be-chromatography. Iqinisekisa iimeko eziguqukayo ngexesha lokwahlukana nokucaca.
I-Synthesis Synthesis: I-MOPS inokuqeshwa njengendawo yokuphinda isebenze okanye inyibilikisi kwi-Synthesis Synthesis. Uzinzo lwayo, ukuluka, kunye neepropathi ze-PH zenze ukuba zilungele ukusetyenziswa kwezicelo ezahlukeneyo zokwenziwa.
Uv Spectrascopy:I-MOPS inolusu olusezantsi kwi-UV phakathi kwe-240-300 NM, iyenza ibe luncedo kwi-UV Spekroscopy yovavanyo. Ivumela imilinganiselo echanekileyo ngaphandle kokuphazamiseka kwi-buffer ngokwayo.
Le yimizekelo nje embalwa yezicelo ezininzi ze-3-morpholinpropanecropanic acid (mops). Ukusetyenziswa kweepropathi zokulawula kunye nezinto zokulawula i-PH kuyenza sisixhobo esibalulekileyo kwizicwangciso ezahlukeneyo zesayensi kunye nophando.
