Iimveliso
Hepes; I-COS.:7365-45-9-9
Izithethantonye: 1- [4- (2-Hydroxyyteyl) -1-i-piperazicacy ye-eshane-2 I-Acid); n- (2-hydroxyytethl) i-piperazine-i-1-ethanesfonic acid; NSCroxyEtheetheethl. NSCroxyEtheetheetheethl. NSCroxyEntyl) I-Acid); N'-2-Hydroxyylpiperachine-I-Ethanesfonic Acid;
Ipropathi yehemicali yehemicanyi
● Inkangeleko / umbala: Umgubo we-crystalline emhlophe
● Uxinzelelo lwe-vapor: 0pa ngo-25 ℃
● Inqaku lokunyibilika: 234-238 ° C
● Isalathiso seNgxelo ye-N20 / D 1.339
● Inqaku lokubila: 408 ℃ [nge-101 325 PA]
● I-PKA: 7.5 (nge-25 ℃)
● psa:I-89.46000
● Ukuxinana: 1.325 g / cm3
● I-LGPP: -0.55930
● Indawo yokugcina i-temp. -
● Ukuzalelwa. :H2O: 1 m nge-20 ° C, icacile, ayinabala
● Ukuzomala amanzi. Uyilo
● I-XlogP3: -4.1
● I-Hydrogen Bop Count Count: 2
● Ubalo lwe-Hydrogen Bopptor: 5
● I-Count Count Count Count: 4
● Ubunzima obuthe ngqo: 238.098728233
● Ukubalwa kweatom enzima: 15
● Ukuntsonkotha: 254
Ulwazi olukhuselekileyo
● I-pictogram (s):
Xi
● Iikhowudi zengozi: xi
● Iingxelo: 36/37/38
● Iingxelo zoKhuseleko: 24/7-22-36-36-26
Iluncedo
I-Canonical iyancuma:I-C1CN (CC [NH +] 1ccs (= o) (= o) [o -]) i-CCO
Inkcazelo:I-Hepes ichazwe njengenye yezona njongo zintle zenjongo ezikhoyo zophando lwebhayoloji. Kwi-phliological PH, imolekyuli yi-zwiterinicic, kwaye isebenza njenge-buffer kwi-PH 6.8 ukuya kwi-8.2 (PKA 7.55). Ihlala isetyenziswa kwinkcubeko yeseli ekugxilwe kuyo phakathi kwe-5mm ukuya kwi-30 mm. I-Hepes isetyenzisiwe kwizicelo ezahlukeneyo, kubandakanya inkcubeko yezicubu. Iqhelekile isetyenziselwa ukuqhubela phambili kwe-Buffer yeNkcubeko yeNkcubeko. U-Hepes ufumana ukusetyenziswa kwawo kwi-vitropents kwi-MG.
IISEBE:Enganetyhefu kwiseli. Isetyenziswa njenge-huffer ye-hydrogen, enokulawula i-PH ye-PH yexesha elide. Uxinzelelo yi-10-50mmol / l. Ngokubanzi, i-20mmol / lhepes kwisisombululo sesondlo sinokufumana amandla e-buffer. Buffer buffer. I-Hepes i-buffer eqhelekileyo yesayensi yebhayoloji, isetyenziswa ngokukodwa kwinkcubeko yeselfowuni ukugcina i-PH. Inika icandelo le-buffring, elisetyenziswa kulungiselelo lwe-buffers. Ichazwa njengenye yezona njongo zilungele ukusetyenziswa kophando lwebhayoloji.
Intshayelelo Eneenkcukacha
HepesImele i-2- (4- (2-Hydroxyytethl) i-piperazin-1-yl) ethanesfonic acid. Iarhente ye-buffer ihlala isetyenziswa kwiibhayibhile zebhayoloji ne-biochemical. Iihempe zinceda ukugcina i-PH ye-PH kwimithombo yeendaba zeselula, njengoko ikwazi ukulesa kwaye inikele iiproteni, ngaloo ndlela ilawula utshintsho. Isakhiwo sayo semichiza kunye neepropathi zenza ukuba kubekho impumelelo kakhulu ekugcineni i-pp engapheliyo nefanelekileyo yokuphendula kwabantu abaninzi bebhayoloji kunye ne-enzymatic. I-Hepes idla ngokusetyenziswa kwinkcubeko, ukuhlanjululwa kweprotein, kunye neendlela zemolekyuli zemolekyuli.
Isicelo
Ii-hepes zinezicelo ezininzi kwimimandla eyahlukeneyo yophando. Ezinye zezicelo eziqhelekileyo zehem zezi:
Inkcubeko yeseli: I-Hepes isetyenziswa njengecandelo kwimithombo yee-sel zenkcubeko yokugcina imeko ye-hle. Inceda ukulawula utshintsho olubangelwa yimisebenzi ye-metabolic kunye nokuqokelelwa kweemveliso zenkunkuma kwiinkcubeko zeseli.
I-Enzyme Assays:Iihedeli zihlala zisetyenziswa njengearhente ye-enzyme Asseys ukugcina i-PH PH PH, ebaluleke kakhulu ekusebenzeni ngokufanelekileyo kwe-enzymes. Inceda ekuqinisekiseni iziphumo ezichanekileyo nezibuyisiweyo.
I-electrophoreris:I-Hepes isetyenziswa njenge-arhente ye-buffer e-electrophoressis ye-Polyacybuslam Inceda ukugcina i-PH yosulelo eMfunayo yokwahlukanisa ngokupheleleyo kunye nohlalutyo lwee-macromolecules ezifana neeprotein kunye ne-nucleic acids.
Ukucoca iiprotein:Ngamanye amaxesha amabandakanyiwe kwi-protein ukucofa i-buffers ukuze alondoloze i-pH enqwenelekayo ngexesha elihlukeneyo lenkqubo yokuphucula. Kuyanceda ukugcina uzinzo kunye nomsebenzi weeproteni ngexesha lokucamngca.
Iindlela zebhayoloji zemolekyuli:I-Hepes inokusetyenziswa kwiindlela ezahlukeneyo zebhayoloji zemolekyuli, ezinjenge-PCR (i-Polymerase Chain) kunye nokulandelelana kwe-DCY, ukugcina isitali esizinzileyo. Inceda ukulungiselela ukusebenza kwee-enzymes kwaye iqinisekisa iziphumo ezichanekileyo nezinokuthenjwa.
Kubalulekile ukuba uqaphele ukuba uxinzelelo oluthile lwehembe kwaye isicelo sayo singahluka ngokuxhomekeka kwiimeko zovavanyo kunye neemfuno.
