Iimveliso
HEPES ityuwa yesodium
Izithethantonye:HEPES ityuwa yesodium;75277-39-3;HEPES hemisodium ityuwa;103404-87-1;Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;sodium 2-[4-(2-hydroxyethyl) ) ipiperazin-1-yl]ethanesulfonate;HEPES (sodium);C8H17N2NaO4S;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, monosodium salt;4-(2-Hydroxyethyl)piperazine-1- ityuwa yesodium ethanesulfonic acid; iSodium 4-(2-hydroxyethyl)piperazin-1-ylethanesulphonate;UNII-Z9FTO91O8A;Z9FTO91O8A;sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate;DTXSID4044458;CHEBI:4675;CHEBI:4675; 278-169-7;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, ityuwa yesodium (1:1);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid,ityuwa yesodium;HEPES isodiyam ityuwa, > = 99.5% (titration); sodium i-hepes;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) ityuwa yesodium;MFCD00036463;HEPES, ityuwa yesodium;HEPES isodiyam ityuwa, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-OPE-S isodium isisombululo.Na; (1M) ibakala;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-0610303839; N'-hydroxyethyl-N-piperazineethanesulfonate;HEPES ityuwa yesodium, i-BioXtra,> = 99.0% (titration);I-HEPES ityuwa yesodium, i-Vetec(TM) ibakala lereagent, 96%;W-104397;Q27120698;Sodium2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;4-(2-Hydroxyethyl) -1-piperazineethanesulfonic acid sodium ityuwa;4-(2-hydroxyethyl)- I-1-piperazineethanesulfonic acid, i-monosodium ityuwa;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)sodiumsalt;N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium ityuwa;HEPES ityuwa yesodium, iBioPerformance Certified, ilungele inkcubeko yeseli, =99.5%
Ipropati yeMichiza ye-HEPES yeTyuwa yeSodium
● Imbonakalo/Umbala:umgubo omhlophe
● Uxinzelelo lomphunga: 0Pa kwi-25℃
● Indawo yokunyibilika:234 °C
● PKA:7.5(ku-25℃)
● PSA:92.29000
● Ubuninzi:1.504[ku-20℃]
● I-LogP: -0.90190
● Ukugcinwa kweTemp.:Gcina eRT.
● Ubuthathaka.:I-Hygroscopic
● Ukunyibilika.:H2O: 1 M ku-20 °C, icacile, ayinambala
● Ukunyibilika kwamanzi.:Kunyibilika emanzini.
● Ubalo lwe-Hydrogen Bond:1
● Ubalo lwe-Hydrogen Bond: 6
● Inani leBond eJikelekayo:5
● Ubunzima obuchanekileyo: 260.08067248
● Ukubala kweAthom Enzima:16
● Ukuntsonkotha:272
Ulwazi oluKhuselekileyo
● IiPictogram:
● Iikhowudi zeeNgozi:
● Iingxelo Zokhuseleko: 22-24/25
Iluncedo
Iiklasi zeMichiza:Olunye Usetyenziso -> Izithinteli zeBiological
I-Canonical SMILES:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Usebenzisa:I-HEPES isisombululo setyuwa yesodium sifanelekile kwizifundo zenkcubeko yeseli. Ingasetyenziswa kulungiselelo lwesithinteli isisombululo se-HEPES. Inokusetyenziswa njenge-buffer ngexesha lovavanyo lwe-phosphorylation kwi-fibroblasts ene-permeabilized. Ingasetyenziselwa ukuqulunqa i-dialysis buffer eyinkimbinkimbi, esetyenziselwa ukuzimisela kwe-thyroxin yamahhala kwi-serum nge-radioimmunoassay. I-HEPES isisombululo setyuwa yesodium sikulungele ukusetyenziswa kwizifundo zenkcubeko yeeseli. Ingasetyenziswa kulungiselelo lwesithinteli isisombululo se-HEPES. Inokusetyenziswa njenge-buffer ngexesha lovavanyo lwe-phosphorylation kwi-fibroblasts ene-permeabilized. Ingasetyenziselwa ukuqulunqa i-dialysis buffer eyinkimbinkimbi, esetyenziselwa ukuzimisela kwe-thyroxin yamahhala kwi-serum nge-radioimmunoassay.
Intshayelelo eneenkcukacha
HEPES ityuwa yesodium, ekwaziwa ngokuba yi-4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium salt, yindlela esetyenziswa ngokuqhelekileyo ye-HEPES. Yikhompawundi ye-zwitterionic ephilayo esebenza njenge-agent ephazamisayo kuphando lwebhayoloji kunye ne-biochemical.
I-HEPES yetyuwa yesodium idla ngokusetyenziswa kwimidiya yenkcubeko yeseli kunye nezithinteli zebhayoloji ngenxa yokukwazi ukugcina uluhlu lwe-pH oluzinzileyo malunga neemeko zomzimba (pH 7.2 - 7.6). Inyibilika kakhulu emanzini kwaye inetyhefu ephantsi, iyenza ilungele usetyenziso olwahlukeneyo.
Ifom yetyuwa ye-sodium ye-HEPES ikhethwa kwizicelo ezininzi njengoko iphakamisa ukunyibilika kunye nokuzinza, xa kuthelekiswa nefom ye-asidi yamahhala. Ifumaneka ngokurhweba njengomgubo omhlophe we-crystalline onokuchithwa lula emanzini ukulungiselela izisombululo zokusebenza.
Abaphandi bahlala besebenzisa ityuwa yesodium ye-HEPES kubuchule bebhayoloji yemolekyuli, inkcubeko yeeseli, uvavanyo lwe-enzyme, ukucocwa kweeprotheyini, kunye nolunye uvavanyo lwe-biochemical apho kubalulekile ukulawula i-pH. Umthamo wayo wokuthintela kunye nokuhambelana neenkqubo zebhayoloji zenza ukuba kube lukhetho oludumileyo lokugcina iimeko ezifanelekileyo zomsebenzi kunye nokuzinza kweeathomli zebhayoloji ezahlukeneyo.
Ngaphambi kokusebenzisa ityuwa yesodium ye-HEPES kuso nasiphi na isicelo esikhethekileyo, kubalulekile ukuphonononga uncwadi, jongana nengcebiso zababoneleli, kwaye ukwandise ugxininiso kunye noluhlu lwe-pH ngokusekwe kwiimfuno ezithile zovavanyo.
Isicelo
I-HEPES yetyuwa yesodium ifumana ukusetyenziswa okuxhaphakileyo kwiindawo ezahlukeneyo zophando lwebhayoloji nakwimichiza. Ezinye zezinto eziphambili zokusetyenziswa zibandakanya:
Inkcubeko yeSeli:Ityuwa yesodium ye-HEPES yongezwa ngokuqhelekileyo kwimidiya yenkcubeko yeseli ukugcina i-pH ezinzileyo ngaphakathi koluhlu lwe-physiological kunye nokubonelela ngokusingqongileyo okufanelekileyo ukuba iiseli zikhule kwaye zande.
Umenzeli wokuBhafa:I-HEPES yetyuwa yesodium isetyenziswa rhoqo njenge-agent ephazamisayo kwizithinteli zebhayoloji kunye nezisombululo, kubandakanywa uvavanyo lwe-enzyme, ukucocwa kweprotheyini, kunye nobuchule bebhayoloji yemolekyuli. Inceda ukugcina i-pH eqhubekayo ngokuchasa utshintsho kwi-acidity okanye i-alkalinity.
Electrophoresis: I-HEPES ityuwa yesodium isetyenziselwa njenge-buffer kwi-gel electrophoresis ukugcina i-pH ezinzileyo ngexesha lokuhlukana kwe-nucleic acids okanye iiprotheni. Ikhusela utshintsho kwi-pH enokuchaphazela ukufuduka kunye nokuhlukana kweepatheni ze-molecule kwi-gel.
Uzinzo lweProtheyini: I-HEPES ityuwa yesodium yongezwa kwizisombululo zeprotheni ukuzinzisa isakhiwo sabo kunye nokugcina umsebenzi wabo. Inceda ukugcina indawo ye-pH eyiyeyona ifanelekileyo kwiprotheyini yozinzo kunye nokusebenza.
Umsebenzi we-Enzyme: I-HEPES yetyuwa yesodium isetyenziswa njengesithinteli kwiintshukumo ze-enzymatic ukugcina i-pH efunekayo ukwenzela umsebenzi we-enzyme. Inceda ukuqinisekisa ukuba ii-enzymes zisebenza ngokufanelekileyo kunye nokuchaneka kwazo.
Umboniso weSeli ebukhoma:I-HEPES yetyuwa yesodium isoloko iqukwa kumajelo emifanekiso ukuze kusetyenziswe umfuniselo we-cell imaging. Umthamo wayo we-buffering unceda ukugcina i-pH efunekayo kwaye uthintele ukuguquguquka okunokuthi kuchaphazele ukusebenza kunye ne-fluorescence yeeseli.
Ubuchule bebhayoloji yeMolekyuli: I-HEPES yetyuwa yesodium isetyenziswa ngokubanzi kwiindlela ezahlukeneyo zebhayoloji yemolekyuli, kubandakanywa i-DNA okanye i-RNA isolation, i-PCR, i-DNA sequencing, kunye nohlalutyo lweprotheyini. Ubume bayo bokugcina buvumela iziphumo ezichanekileyo nezithembekileyo ngexesha lezi nkqubo.
Kubalulekile ukuqaphela ukuba usetyenziso oluthile kunye noxinzelelo lwetyuwa yesodium ye-HEPES inokwahluka ngokuxhomekeke kwiimfuno zovavanyo kunye nenkqubo yebhayoloji ephantsi kophononongo. Ke ngoko, kuyacetyiswa ukuba uqhagamshelane noncwadi kunye neengcebiso zababoneleli ukuze usetyenziswe ngokugqibeleleyo.
