Iimveliso
I-Hepes sodium yetyuwa; I-CASS. I-CAS: 75277-39-3
Izithethantonye: Hepes Sodium ityiwa; 75277-39-39; Heis Hemimim Hemimozin)-(i-SODIim 2- (2-yl] ethanesfonate; Hepes (isodium); c8h17n27N2S; ityiwa; 4- (2-hydroxyytethl) piperazine-1-ethanesfoni I-4- (2-HydroxyEtheetheetheetheethl)-1-Ylenela81o81o8a; I-Chebi: I-Einecs I-278-169-7; i-1-piperazineethanesline Hepes; n- (2-hydroxyytethl) i-piperazine-n '- (2-ethanesfonic acid); i-MFCD00036463; I-Hepes, ityiwa ye-sodium, I-98%; c8h18n2N2O4S.NA; I-SCTZLL21872: I-CHERZYID20-V2 . Ibakala; I-Akos01589769; Akos0151222; I-Akos0151103; I-Cs-061086; I-AD-038366; I-AD-038366; I-AD-038366; I-AD-038366; I-AD-038366; I-AD-038366; I-AD-038366; I-AD-038366; I-A8383 I-N'-hydroxyylyl-n-piperazineethanese; Hepes Sodium, Bictra,> = i-99.0% I-96%; W-104397; Q2710698; I-Sodium2- (2- (2-Hldroxineetheethl ityiwa; 4- (2-hydroxyythl) -1-i-piperazium ityiwa; hepes sodium yetyuwa, iqinisekisiwe eqinisekiswe, ilungele inkcubeko yeselfowuni,> = 99.5%
Ipropathi yekhemikhali ye-Hepes Sodium Ityiwa
● Inkangeleko / umbala: ipowder emhlophe
● Uxinzelelo lwe-vapor: 0pa ngo-25 ℃
● Inqaku lokunyibilika: I-234 ° C
● I-PKA: 7.5 (nge-25 ℃)
● psa:I-92.29000
● Ukuxinana: 1.504 [nge-20 ℃]
● I-LGPP: -0.90190
● Indawo yokugcina indawo.
● Ngokutsho
● Ukuzalelwa. :H2O: 1 m nge-20 ° C, icacile, ayinabala
● Ukuzola amanzi
● I-Hydrogen Bop Count Count: 1
● Ubalo lwe-Hydrogen Bopptor: 6
● I-COCT ROCTAT: 5
● Ubunzima obuthe ngqo: 260.08067248
● Ukubalwa kweatom enzima: 16
● Ukuntsonkotha: 272
Ulwazi olukhuselekileyo
● I-pictogram (s):
● Iikhowudi zengozi:
● Iingxelo zoKhuseleko: 22-24 / 25
Iluncedo
Iiklasi zekhemikhali:Olunye usetyenziso -> I-buffers yebhayoloji
I-Canonical iyancuma:I-C1CN (CCN1CCO) ccs (= o) (= o) [o) [o). [E +]
IISEBE:Isisombululo se-Hepes sodium silungele izifundo zenkcubeko yeselfowuni. Ingasetyenziswa kulungiselelo lwe-buffer ye-hepes. Ingasetyenziswa njenge-buffer ngexesha le-phosthory yokudumba kwi-fibroblied fibroblasts. Ingasetyenziselwa ukuqamba i-buffer entsonkothileyo yediski, esetyenziselwa ukumiselwa kwe-thyroxin yasimahla kwi-serum nguRaum nguRaum msonomimmunosay. Isisombululo se-hepes sodium silungele ukusetyenziswa kwizifundo zenkcubeko yeselula. Ingasetyenziswa kulungiselelo lwe-buffer ye-hepes. Ingasetyenziswa njenge-buffer ngexesha lokudumba kwe-phosthorys kwi-fibroblealid fibroble. Ingasetyenziselwa ukuqamba i-buffer entsonkothileyo yediski, esetyenziselwa ukumiselwa kwe-thyroxin yasimahla kwi-serum nguRaum nguRaum msonomimmunosay.
Intshayelelo Eneenkcukacha
Hepes sodium ityiwa, ekwabizwa ngokuba yi-4- (2-hydroxyytethl) i-piperazine-1-ethanesfonis Acid Sodium, yindlela eqhelekileyo esetyenziswayo. Yindawo ye-sellricion ye-zeiterinic esebenza njengearhente ye-buffer kwi-biologizy kunye ne-biochemical up.
I-Hepes Sodium ihlala isetyenziswa kwimithombo yeedolophu kunye ne-buffers yebhayoloji ngenxa yokukwazi ukugcina iimeko ezizinzileyo (PH 7.2 - 7.6). Inyibilikisiwe kakhulu emanzini kwaye inetyhefu ephantsi, iyenza ilungele izicelo ezahlukeneyo.
I-sodium yemotyi ye-hespes yokhethwa kwizicelo ezininzi njengoko ikhuthaza ukuntywila nokuzinza, ngokuthelekiswa nefom ye-acid yasimahla. Ifumaneka ngorhwebo njengeplestalline emhlophe enokutshitshiswa ngokulula emanzini ukuze balungiselele izisombululo zomsebenzi.
Abaphandi bahlala besebenzisa i-hepes sodium ye-molecular yobugcisa be-molecular, inkcubeko, i-enzyme Adushs, ukuhlanjululwa kweproteyini, kunye nezinye ii-biochemical apho ulawulo lubalulekile khona. Umthamo wayo we-buffiting kunye nokuhambelana neenkqubo zebhayoloji zenza ukhetho oluthandwayo lokugcina iimeko ezifanelekileyo zomsebenzi kunye nokuzinza kweemolekyuli ezahlukeneyo zebhayoloji.
Ngaphambi kokuba basebenzise ityiwa ye-Hell Sodium nakwesiphi na isicelo esithile, kubalulekile ukuphonononga iingcebiso, nxibelelana nabathengisi, kwaye uqhubekele ukugxininisa kwiimfuno zovavanyo oluthile.
Isicelo
I-Hepes Sodium ifaka isicelo ngokubanzi kwiiNdawo ezahlukeneyo zebhayoloji ne-biochemical. Ezinye zezinto zayo eziphambili zibandakanya:
Inkcubeko yeseli:I-Hepes Sodium ixhaphane inkcubeko yamajelo eesetimedi ukugcina i-PH ye-PH ye-PH ngaphakathi kudidi lwezinto zomzimba kwaye ibonelele ngendawo efanelekileyo yeseli ekhule kwaye indala.
Iarhente ye-Bufferty:I-Hepes Sodium idla ngokuqeshwa njengearhente ye-buffer buffers kunye nezisombululo, kubandakanya ukufunyanwa kwe-enzyme, ukucocwa kweprotein, kunye neendlela zemolekyuli. Inceda ukugcina i-PH ye-PH ngokuxhathisa iinguqu kwi-acidity okanye ialkalinity.
I-electrophoreris: I-Hepes sodium ityiwa njenge-buffer kwi-gel Electrophoressis ukugcina i-Ph Ph ngexesha lokwahlula i-acid yenyukliya okanye iiproteni. Ithintela utshintsho kwi-PH enokuthi ichaphazele ukufuduka kunye neepateni zokwahlula kweemolekyuli kwijeyi.
Uzinzo lweprotheyini: I-Hepes Sodium yongezwa kwiZisombululo zeProtein zokuzinzisa isakhiwo sabo kwaye igcine umsebenzi wazo. Inceda ukugcina imeko ye-ph ifanelekileyo eyimfuneko ukuze uhlale uzinzo kunye nokusebenza.
Umsebenzi we-enzyme: I-Hepes sodium ityiwa njenge-buffer yokuphendula kwi-enzymatic yokugcina umsebenzi ofunekayo we-enzyme. Inceda ekuqinisekiseni ukuba imisebenzi e-enzymes emsebenzini iphezulu kwaye ichanile.
I-Lial Comple Inet:I-Hepes Sodium idla ngokuqukwa kwimidiya yokucinga yeendlela zokuvavanya ifowuni. Umthamo wayo we-buffring unceda ukugcina i-PH kwaye ithintela ukuguquguquka okunokuchaphazela ukusebenza kunye nokuguquguquka kweeseli.
Iindlela zebhayoloji zemolekyuli: I-Hepes Sodium iqeshwe ngokubanzi kwiindlela ezahlukeneyo zemolekyuli, kubandakanya iDNA okanye i-DNA yodwa, i-PCR, i-DCE, kunye nohlalutyo lweproteyini. Amandla ayo e-bufffding ivumela iziphumo ezichanekileyo nezinokuthenjwa ngexesha le nkqubo.
Kubalulekile ukuba uqaphele ukuba isicelo esithile kunye nokugxilwa kwetyuwa ye-Hepes Sodium kungahluka ngokuxhomekeka kwiimfuno zovavanyo kunye nenkqubo yebhayoloji phantsi kovavanyo. Ke ngoko, kuyacetyiswa ukuba uqwalasele noncwadi kunye neengcebiso zabasebenzi zokusetyenziswa ngokugqibeleleyo.
