Iimveliso
Hepps; I-CAS: 16052-06-5
Izithethantonye: Hepps; 16052-06-5; i-epps; I-ACID; 3- [4- (2-Hydrozinyl] -1-i-1-piperazinethl) - i-1- yl] i-piperazi-1-ilfoning I-acid; n- (2-hydroxyytethl) i-piperazine I-240-198-8; BrN 3957385; I-8jn5pqw4pqw4pkw i-1- I-acid; ep1; n-2-hydroxyylpiperachine-n-3-propanesis . I-99%; AC-24630; AS-69273; I-EPPS, Biolotra,> = 99.5% (izivakalisi); ls-113260; CS-0015183; ft-06165; I-acid; n-2-hydroxyylpiperachine-i-propanesfonic acid; Q15635; (4- I-ACID; 4- (2-Hydroxyytethl) i-piperazine-1-I-Acid-SulDroxythyl); (Hepps);
Ipropathi yehemicali yehemicaphu
● Inkangeleko / umbala: Umgubo we-crystalline emhlophe
● Inqaku lokunyibilika: 237-239 ° C (ikhanyisiwe.)
● Isalathiso seNgxelo yeNgxelo: 1.537
● I-PKA: 8.0 (nge-25 ℃)
● psa:I-89.46000
● Ukuxinana: 1.285 g / cm3
● I-LGPP: -0.16920
● Indawo yokugcina indawo.
● Ukuzalelwa. :H2O: 1 m nge-20 ° C, icacile, ayinabala
● Ukuzola amanzi 25.2 g / l (20 ° C)
● I-XlogP3: -3.6
● I-Hydrogen Bop Count Count: 2
● Ubalo lwe-Hydrogen Bopptor: 6
● I-Count Chot Rot: 6
● Ubunzima obuthe ngqo: 252.11437830
● Ukubalwa kweatom enzima: 16
● Ukuntsonkotha: 280
Ulwazi olukhuselekileyo
● I-pictogram (s):
Xi
● Iikhowudi zengozi: xi
● Iingxelo: 36/7/8-222
● Iingxelo zoKhuseleko: 24 / 25-36-26-26
Iluncedo
Iiklasi zekhemikhali:I-Nitrogen Compows -> Piperazines
I-Canonical iyancuma:I-C1CN (CCNCCcs (= o) (= o) o) i-CCO
IISEBE:I-EPPS lisetyenziswa ngokufuthi isetyenziselwa i-buffer elungileyo. Ihlala isetyenziswa njengesahluli kwi-iltrathiric slolectric egxile kwaye iphucule isisombululo se-phosphogluase. Isetyenziswa njengearhente ye-byfffrity kwi-biology kunye ne-biochemistry.
Intshayelelo Eneenkcukacha
I-ECPS imele i-N- (2-hydroxyythl) i-piperazine Yindawo ye-wittieridic ye-zeftitidic esetyenziswa rhoqo kwi-phailograficals kunye ne-bichemical bophaeli.ip yophando.
Iipropathi ze-Bufffrity:I-EPPS yiarhente esebenzayo esebenzayo enceda ukugcina i-PH ye-PH ekusetyenzisweni kwezilingo. Inexabiso le-pka le-8.09, eyenza ilungele ukutshitshiswa kwi-PH Uluhlu lwe-7.0-9.0.
Uzinzo:I-EPPS ibonisa uzinzo oluhle ngaphezulu koluhlu lwamaqondo obushushu kwaye alichanekanga kukutshintsha kobushushu xa kuthelekiswa nezinye iiarhente ze-ris kunye ne-phosphate buffers. Olu inzo luyenza ilungele ukusetyenziswa kwiimvavanyo ze-biochemical kunye nezendalo ezifuna iimeko ezilawulwayo.
Izicelo zebhayoloji:Ii-EPPs zihlala zisetyenziswa kwizifundo ezahlukeneyo zebhayiloji, ezinjengokuhlanjululwa kweProtin, i-Enzyme Adusays, kunye neDNA / Rona. Umthamo wayo wokutshixiza kunye nozinzo kwi-phlilogical ph ibaluleke kwezi zicelo.
Inkcubeko yeseli:I-EPPs inokusetyenziswa njengearhente ye-buffer kwi-Intuth ye-sel yeselula. Inceda ukugcina imeko emiZinzayo ilungele ukukhula kunye nokugcinwa kweentlobo zeseli ezithile.
I-IYO kunye nokuhambelana:I-EPPS yindawo ye-zeittiericic, intsingiselo ineendleko ezintle nezingalunganga. Eli nqaku liyayivumela ukuba iqhubele phambili i-buffer zombini izisombululo ze-acidic kunye nezisiseko. Ihambelana neemolekyuli ezahlukeneyo zebhayoloji kwaye ziyaziwa ukuba ziphazamiseke kancinci.
Xa usebenzisa i-EPPs, kubalulekile ukulandela izikhokelo ezicetyiswayo kunye nezikhokelo ezibonelelwe ngumenzi. Ukongeza, hlala ulumkele kwaye ulandele iiprothokholi zokhuseleko ezifanelekileyo xa uphatha nayiphi na iarhente yekhemikhali okanye iarhente ye-buffer.
Isicelo
Hepps (n- (2-hydroxyytethl) i-piperazine-n-3-propasulfonic acimec) yi-biiterinic buffer esetyenziswayo kuPhando lwebhayoloji ne-biochemical. Ezinye zezi nkqubo zayo zibandakanya:
Inkcubeko yeseli: I-hepps isetyenziswa njengearhente ye-buffer kwi-Counel Inkcubeko yokugcina i-PH iqhubeka kwaye inike imeko efanelekileyo yokukhula kweseli nokwanda. Inceda ukuzinzisa i-PH ngaphakathi kwe-PH Uluhlu lwe-deiological kwaye kuthintele nayiphi na imiphumo emibi kwiinkqubo zeselula.
I-Enzyme Assays:I-hepps ihlala isetyenziswa njenge-buffer kwi-enzyme Asseys yokwandisa iimeko ze-ps eziyimfuneko kumsebenzi ophezulu we-enzyme. Umthamo wayo we-buffring unceda ukugcina izitali ze-PH, ukuqinisekisa imilinganiselo echanekileyo ye-enzyme.
Ukucoca iiprotein:I-hepps inokusetyenziswa njenge-buffer kwiinkqubo zokucoca iiprotein. Kuyanceda ukugcina uzinzo kunye nomsebenzi weeproteni ngexesha lokucamngca, ezinjengeekholamu chromatography okanye ukucofa iprotein kusetyenziswa iithegi ze-fats.
I-electrophoreris:I-hepps ngamanye amaxesha isetyenziswa njenge-buffer kwi-gel Electrophoressis yokwahlula kunye nohlalutyo lweeprotein kunye ne-procleic acids. Umthamo wayo we-PH unceda ukugcina i-PH eyenziwayo ye-elektroniki, inzale kwi-molekyuli echanekileyo.
Iindlela zebhayoloji zemolekyuli:I-hepps inokusetyenziswa njenge-buffer kwiindlela ezahlukeneyo zebhayiloji zemolekyuli, kubandakanya i-PCR (i-Polymerase Chain), ukulandelelana kwe-DCE, kunye nokuphendula kwe-edlymatic. Isakhono sayo esibukhali siqinisekisa ukuba iimeko ezilungileyo ze-PH zokuphendula okuphumelelayo kunye nokusebenza ngokufanelekileyo.
Izifundo ezibandakanya iiproteri ze-membrane:I-hepps ilungele ukufundela iiproteni ze-membrane ngenxa yepropathi ye-membrane ephantsi. Ihlala isetyenziswa njenge-buffer kwiimvavanyo ezibandakanya ubonakaliso lwezinto ezisebenzayo okanye ngokutsha kweeproteri ze-membrane.
Kubalulekile ukuba uqaphele ukuba uxinzelelo olufanelekileyo kunye ne-PH ye-hepps inokuhluka ngokuxhomekeke kwisicelo okanye kwiimeko zovavanyo. Ke ngoko, kuyacetyiswa ukuba uqwalasele noncwadi okanye izindululo zabasebenzi zokusetyenziswa okufanelekileyo kunye nokugxininiswa kwe-hepps kwiimvavanyo zakho.
