Iimveliso
Ityuwa ye-mopso ye-mopso; I-CAS: 79803-73-9
Izithethantonye: I-4-Morphonelineprojeulfoulfonidid, i-b-hydroxy-, i-monosodium (9CI)
Ipropathi yamachiza ye-mopso sodium ityiwa
● Pka: 6.9 (nge-25 ℃)
● psa:I-98.28000
● I-LGPP: -0.75660
● Indawo yokugcina indawo.
● Ukuzalelwa. :H2O: 1 m nge-20 ° C, icacile, ayinabala
Ulwazi olukhuselekileyo
● I-pictogram (s):
Xi
● Iikhowudi zengozi: xi
● Iingxelo: 36/37/38
● Iingxelo zoKhuseleko: 26-36
Iluncedo
IISEBE:I-Mopso Sodium I-Buffer ye-Biological ijongene nesizukulwana sesibini "i-buffer" entle "ebonisa ukusonjelwa kokubizwa kwabo kuthelekiswa ne-buffers yemveli" entle ". I-PKA ye-Mopso Sodium yi-6.9 eyenza ukuba ibe ngumgqatswa ofanelekileyo wefomu le-buffer efuna i-PH ubuncinci iphantsi ukugcina imeko yezinzile kwisisombululo. I-Mopso Sodium ithathwa njengeyinto engenatyhefu kwimigca yenkcubeko kwaye ibonelela ngokucalula isisombululo .mopso sodium inokusetyenziswa kwimithombo yeendaba ye-Biophapharte (zombini i-biopharmam) kunye ne-Dowstream) kunye ne-Dowstream) kunye ne-Dowstream. Ii-Buffers ezisekwe kwi-Mopso zichazwe ngokulungiswa kweeseli kwiisampulu zomchamo.
Intshayelelo Eneenkcukacha
I-mopso sodium ityiwa, ekwabizwa ngokuba yi-sodium 3- (N-Morpholino) propanondate, yi-buffer esetyenziswayo esetyenziswayo kwi-biologizy kunye ne-biochemical up. Yinto yomgubo we-crystalline emhlophe enyibilikayo emanzini. Ityuwa ye-mopso sodium ihlala isetyenziswa njenge-buffer ukugcina ixabiso elizinzileyo kwiimvavanyo ezahlukeneyo zebhayoloji kunye nokuphendula okungekho mthethweni. Iluncedo ngakumbi kwizicelo ezifuna i-6.5 ukuya kwi-7.9 ngenxa yexabiso layo le-7.2. Olu luhlu lwe-buffer lwenza lufanelekile inkcubeko yeseli, ukuhlanjululwa kweprotein, kunye neendlela zemolekyuli zemolekyuli.
Ukongeza kumthamo wayo we-buffir, i-mopso sodium ityiwa kukwazi ukuzinzisa iiproteni ezithile kunye nee-enzymes, ukunceda ukugcina umsebenzi wabo kunye nesakhiwo sazo. Ithathwa njenge-lufstinic buffer, intsingiselo inokubakho kwiifom ezifanelekileyo nezihlawulelwayo, kuxhomekeka kwi-PH yesisombululo. Xa usebenzisa ityiwa ye-mopso sodium, kubalulekile ukuba ulinganise kwaye ulungiselele izisombululo ze-buffer ngokuchanekileyo ukufezekisa inqanaba le-ph enqwenelekayo. I-ten meter meterm meter okanye isalathiso se-hle siyacetyiswa ukuba sibeke esweni kwaye sihlengahlengise i-PH ngokufanelekileyo.
Lilonke, i-Mopso Sodium sisixhobo esixabisekileyo kuphando lwelebhu, ukubonelela ngendalo esizinzileyo kunye nokuxhasa iimvavanyo ezahlukeneyo zebhayoloji ne-biochemical.
Isicelo
I-Mopso Sodium Ityiwa (3- Nazi ezinye iindlela apho ityiwa ye-Morko Sodium inokuba yingenelo:
Iarhente ye-Bufferty:Ityuwa yeMopso Sodium iqhele ukusetyenziswa njengearhente ye-buffer kwiimvavanyo ezahlukeneyo zebhayoloji kunye ne-biochemical. Inceda ukugcina i-PH ye-PH ye-PH, ebaluleke kakhulu kwimisebenzi ye-enzyme efanelekileyo, uzinzo lweprotheyini, kunye nezinye iinkqubo zebhayiloji.
Iprotein Crystallization:Ityuwa ye-mopso sodium ihlala isetyenziswa kwizikrini zokukhaba zeProtein ukwenza ukhuselo lwekristali ephezulu yeprotein. Umthamo wayo ophosakeleyo unceda ukugcina i-PH eguqukayo, ebaluleke kakhulu kuzinzo nolwakhiwo lweekristasi zeprotein.
I-electrophoreris:I-mopso sodium iyo isebenza njengecandelo lobuchule lobuchule njenge-SDS-Page (SODIium DODCYL SULDCY-POLYCY SLEPROPHISIR) Isetyenziselwa ukwahlulahlula kunye nokuhlalutya iiproteni. Iipropathi zayo ezi-buffrid zinceda ukugcina i-PH PH, iqinisekisa ukwahlulwa kweprofayili kunye nohlalutyo.
I-Enzyme Assays:I-mopso sodium ityiwa iluncedo kwi-enzyme Adus kunye nezifundo ze-kinetic, apho kunceda ukugcina ukugcina izinzile nolawulwa khona. Oku kubalulekile kumlinganiso ochanekileyo womsebenzi we-enzyme kunye nokuqonda iiparameter ze-kinetic.
Isisombululo se-biochemical:Ityuwa ye-Mopso Sodium isetyenziswa ekuqulunweni kwezisombululo zebhayiloji, ezinje nge-buffers zeprotheni, ukucocwa kunye nokugcinwa. Isikhundla sayo esikrwitshi siqinisekisa ukuba i-PH ihleli iqhubeka, ukunciphisa ukwahluka kwezinto zeprotein kunye nokusebenza.
Kubalulekile ukuba uqaphele ukuba uxinzelelo oluthile kunye neemeko ze-mopso sodium isutium ingenisayo kuxhomekeke kuvavanyo oluthile okanye isicelo. Soloko ubhekisa kwiiprothokholi ezifanelekileyo kunye nezikhokelo zokulungiselela ezifanelekileyo kunye nokusetyenziswa kwetyhubhu ye-mopso sodium kwilingo lakho.
