Iimveliso
Imibhobho; I-CAS: 5625-3-6-6
Izithethantonye:I-1,4-piperazeneIeneiethane Sulfonic Acid;
I-1,4-piperazeneightphonenenenenenec acid;
I-1,4-piperazineethaneulrhane; i-piperazine-n, bis (2-ethanesfoninininininininion);
I-piperazine-n, zula (2-ethanesfonic acid), ityiwa yesodium; imibhobho
Ipropathi yemibhobho yemibhobho
● Inkangeleko / umbala: I-White / Ecricalline Powder
● Inqaku lokunyibilika:> I-300 ° C (ikhanyisiwe.)
● Isalathiso sokuqaqamba: 1.6300 (Qikelela)
● Pka: 6.8 (nge-25 ℃)
● I-PSA: 131.98000
● Ukuxinana: 1.505 g / cm3
● I-LOGP: 0.41700
● Indawo yokugcina indawo.
● Ukuzithoba .:0.1 M NaYoH: 0.25 g / ml, icacile, ayinabala
● Ukuzola amanzi
● I-XlogP3: -6.7
● I-Hydrogen Bop Count Count: 2
● Ubalo lwe-Hydrogen Boptor: 8
● I-Count Chot Rot: 6
● Ubunzima obuthe ngqo: 302.06062865
● Ukubalwa kweatom enzima: 18
● Ubunzima: 397
Ubunyulu / umgangatho
I-99% * Idatha evela kubathengisi be-Raw
I-1,4-piperazinebis (i-ethanesfounidid) 99% * Idatha evela kubathengisi be-Regement
Ulwazi olukhuselekileyo
● I-pictogram (s):
Xi
● Iikhowudi zengozi: xi
● Iingxelo: 36/37/38
● Iingxelo zoKhuseleko: 22-24 / 25-37 / 39-26
Iifayile zeMSDS
Iluncedo
Iiklasi zekhemikhali:Olunye usetyenziso -> I-buffers yebhayoloji
I-Canonical iyancuma:I-C1CN (CCNCS (= o) (= o) o) ccs (= o) (= o) o
IISEBE:I-piperazine-n bis- (i-chais- (i-2-ethanespholphoal acid) i-ultra-i-59.5%) yi-TORMEDIIA ebalulekileyo kuzo zonke iintlobo zekhemikhali. I-1,4-piperazidiethanesfoniethanenion, i-piperazine-n, i-chan- (i-chanesfoul acid isebenzisa isisombululo se-biologion esebenzisa isisombululo se-biellional) Iarhente ye-buffrity ene-PKA kufutshane ne-ph.pipes [i-piperazine-n, bis (2-ethanesfonic acid) isetyenziswa njengearhente ye-biochemistry. Yi-ethanesfoul buffer buffer iphuhliswe yi-et elungileyo et al. Ngo-1960s. Imibhobho ine-pka kufutshane ne-phOlogical P eyenza iluncedo kumsebenzi wenkcubeko. Ibhalwe ukuba inciphise ilahleko ye-gipid i-giputaraldehyde ye-tistology kwi-tissus ye-tity kunye nezicubu zeZilwanyana. Imibhobho ye-ripessium ye-dipes; Imibhobho yeTyudisi ityiwa; Imibhobho, ityiwa yesodium. I-Buffers inokulungiswa ngokongeza isisombululo semibhobho ye-acid yasimahla, ishumi kwi-PH efanelekileyo, okanye ngokuxuba isisombululo setyuwa ye-monosodium kunye netyuwa ye-PH. Imibhobho, isetyenziswa iarhente ye-byffist kwi-biochemistry. Imibhobho inayo i-pka (6.76 nge-25 ° C) kufutshane ne-phOrilogicalulogical P eyenza iluncedo kumsebenzi wenkcubeko. Imigaqo-nkqubo ibilisiwe ekusebenziseni imibhobho yokwahlulahlula i-glyculoted ye-Glysoxydilated RNA kwi-agarose Gels, i-NUCLEAE S1 yeRNA, naseRibonueClease SyAy Croptols. Imibhobho isetyenzisiwe njenge-buffer kwi-glutaraldehde yokulungisa iisampulu ze-tishu Imibhobho icetyisiwe ukuba isetyenziswe kwi-buffers ye-vitro yokufunda kwi-CASPAASS 3, 6, ne-8. Isifundo esipapashiweyo sabonisa ukuba siluncedo kwimibhobho ye-ion ye-ion ye-ion enjalo. Imibhobho isetyenziswe kwinkcubeko yeselfowuni yezicelo ezinjengeenjineli ye-membrane ye-membrane e-Escherichichia Coli.
Intshayelelo Eneenkcukacha
Imibhobho ye-acid yasimahla, ekwabizwa ngokuba yi-1,4-piperazeeiethaneimeneninion acid, i-buffer elungileyo iphawuleka ukuba inexabiso le-pka le-PKA ye-PC. Ngenxa yoko, imibhobho yasimahla isetyenziswa rhoqo njenge-buffer kuphando lwe-biochemical.mipes yi-zwitterion, i-piperazinic buffer eluncedo kwi-PH ye-6..1 - 7.5. Imibhobho ingenayo amandla okwenza ubunzima obubalulekileyo ngee-ion ezininzi zentsimbi kwaye ziyacetyiswa ukuba zisetyenziswe njenge-buffer engalungelelanisiweyo kwizisombululo zentsimbi. Imibhobho yeentlobo ngeentlobo zeentlobo kwaye iqhele ukusetyenziswa kwimidiya yenkcubeko, kwi-protery ye-protery, njenge-buffer esebenzayo kwi-gel Electrophoresis, kwaye njenge-shromatography. Le buffer ikwazi ukwenza i-radicals kwaye ke ayifanelekanga ukuba i-Redoption Reaction. Ilungele ukusetyenziswa kwi-bicinchoninic acid (BCA). I-Soling yemibhobho inyuka xa i-acid yasimahla iguqulwa kwi-sodium ye-sodium.
Imibhobho lilungu le-ethanesfonic acid buffer uthotho, yaziswa kwi-ET CATE. Ukusukela i-PKA yayo kwi-37 ° C ikufuphi ne-PH ye-PH, imibhobho ineenkqubo zomsebenzi wenkcubeko.
Intshayelelo Eneenkcukacha
Imibhobho, ekwabizwa ngokuba yi-piperazine-n, bis (2-ethanesfonic acid), yi-buffer acid), yi-buffer acid esetyenziswayo kwi-biologiolo phanda yophando. Yindawo ye-yewittiericic enceda ukugcina izizinzo zeZisombululo eziMvume.
Enye yezicelo eziphambili zemibhobho kwintsimi ye-molecular kunye ne-biochemistry, apho isetyenziswa khona njenge-buffer yokulungiselela nokugcina iisampulu zebhayoloji. Imibhobho yexabiso le-pka malunga ne-7.46, eyenza ilungele ukugcina i-6.7 ukuya kwi-7.9 kwiqondo lobushushu (malunga ne-37 degrees Celsius).
Imibhobho iluncedo ngokukhethekileyo ekugcineni i-PH PH enjengeenkqubo ezinjengokucocwa kweprotheni, i-encymes, kunye ne-gel electrophoreris. Kuyanceda ukunciphisa utshintsho lwe-pH olunokuthi luchaphazele ubume kunye nomsebenzi weeproteni kunye nee-enzymes.
Olunye ukusetyenziswa kwemibhobho kuphando lwenkcubeko yeselfowuni. Iqhelekile isetyenziswa njenge-buffer yemithombo yeeMgadi zeNkcubeko. Imibhobho iyanceda ukugcina i-PH PH PH isebenza phakathi, ebaluleke kakhulu ekukhuleni nasekusebenzeni kweeseli. Ikwabonelela ngendawo esizinzileyo yokufunda iinkqubo zeselula kunye nokwenza uvavanyo.
Imibhobho ikwasetyenziswa kwiindlela ezahlukeneyo zokuhlalutya, njenge-chromatography ye-chromatography kunye ne-capillary elektrophoressis. Umthamo wayo we-buffiting kunye nozinzo ze-PH zenza ukuba zilungele ukusetyenziswa kwezi ndlela, apho ulawulo lwe-PH lubalulekile kwiziphumo ezichanekileyo.
Ukongeza, imibhobho ifumene izicelo kwiinkqubo zokuqulunqwa kweziyobisi kunye neenkqubo zokuhambisa. Ingasetyenziswa njenge-buffer kwifom yamayeza yokwandisa uzinzo lweziyobisi kunye nokuphucula ukufunwa ngamachiza.
Ngokubanzi, imibhobho yiarhente ye-butffiley eguquguqukayo enoluhlu olubanzi lwezicelo kuPhando lweNdalo kunye neBiochemical, inkcubeko ye-biechemical, iindlela zokufumana uhlalutyo. Isakhono sayo sokugcina isizinzo senziwe sisixhobo esibalulekileyo kwimimandla eyahlukeneyo yesayensi.
